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KMID : 0613319990050020087
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Journal of the Korean Bone and Joint Tumor Soceity
1999 Volume.5 No. 2 p.87 ~ p.94
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A Study of Bone Healing in Irradiated Autogenous Bone Grafting
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Chae Jong-Min
Park Hee-Kyung
Lee Byung-Yook
Suh In-Soo
Suh Kyung-Jin
Park Il-Hyung
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Abstract
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To evaluate the efficacy of demineralization In bone healing of irradiated recycled autogenous bone graft, light microscopy, immunohistochemistry and electron microscopy studies were carried out. Segmentally rejected rabbit specimens with the periosteum attached, 1.5cm in length from the radius, were demineralized by the treatment with 0.6 N HCl after 5,000 cGy irradiation and used as recycled autografts. The histological changes were examined at 1 and 2 weeks, also at 1, 2, 4, and 6 months after reimplantation. The immunohistochemical reactions of osteonectin, osteocalcin, and TGF-βl were also evaluated. In the early stages, 1 week after recycled grafting with irradiation and no other treatment, all osteocytes in the graft were degraded and necrotized. The compact proliferated cells aggregated around the recycled bone showed a positive reaction in osteonectin and TGF-βl, but a negative reaction in osteocalcin. Osteoclastic activity was not prominent. At 2 weeks, small amounts of cartilage were formed, and with the increase of new bone the graft materials were absorbed. At 4 months, irradiated recycled autograft without any other treatment replaced new bone and was incorporated in the host. In the group of recycled autografting after demineralization following irradiation, after 1 week, osteoclastic activity on the graft was minimal and negative to osteocalcin reaction. After 2 weeks, cartilage had formed around the recycled bone and differentiated chondrocytes were noticed in the old vascular space and lacuna of the graft, as well as the production of cartilaginous matrices. These cartilagenous matrices within the reimplanted cortical bone were gradually replaced by new bones. At 3 months after recycled autografting, fusion occurred between the graft and host bone. The result suggested that demineralization in bone healing of irradiated recycled autogenous bone graft lowered osteocalcin expression and inhibited absorption of the reimplant and induced endochondral bone formation. The suppression of osteocalcin effects may be helpful in the healing of recycled irradiated bone.
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KEYWORD
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Autogenous bone grafts, Irradiation, Demineralization, Bone Proteins
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